Electrophoretic Analysis of Candida albicans Isolates Collected From Turkish Hospitals by Native- Polyacrylamide Gel Electrophoresis (N-PAGE)

نویسندگان

  • Özlem OSMANAĞAOĞLU
  • Sefa C. SAÇILIK
  • Ufuk GÜNDÜZ
  • Nurten ALTANLAR
  • Cumhur ÇÖKMÜŞ
چکیده

Native-polyacrylamide gel electrophoresis (N-PAGE) was used to characterize Candida albicans strains isolated from vaginal specimens of patients suffering from vaginitis in Ankara hospitals, Turkey. Three different protein band profiles were observed among these 38 C. albicans isolates when whole cell protein extracts of these strains were used in N-PAGE. 34 of the 38 isolates were shown to belong to a single group or clone with a dissemination percentage of 89, while three isolates belonged to another group with a percentage of 8. Furthermore only one C. albicans isolate was detected with a dissemination percentage of 3 and assigned to a third group. The results presented here also suggested that N-PAGE may be an efficacious method for differentiating between C. albicans strains. Türk Hastanelerinden Toplanmış Olan Candida albicans İzolatlarının Native-Poliakrilamid Jel Elektroforez ile Elektroforetik Analizi Özet: Ankara’daki hastanelerde vajinit problemli hastalardan toplanmış olan 38 adet Candida albicans suşu native-poliakrilamid jel eletroforez (N-PAGE) ile karakterize edilmiştir. C. albicans izolatlarının hücre protein ekstraktları N-PAGE’de kullanıldığı zaman üç değişik protein bant patternine rastlanmıştır. Bu çalışmanın sonucunda toplam 38 C. albicans izolatının 34’ünün yayılım oranı %89 olarak tespit edilen tek bir gruba veya klona ait olduğu belirlenmiştir. Diğer taraftan % 3’lük bir yayılım oranına sahip olan tek bir C. albicans suşu tespit edilip üçüncü bir gruba veya klona ait olduğu belirlenmiştir. Bu çalışma sonucu elde edilen veriler N-PAGE’nin C. albicans suşları arasında ayırım sağlayacak düzeyde etkili bir metod olabileceğini önermektedir.

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تاریخ انتشار 2000